Changes in Free and Esterified Cholesterol

Mechanism of cholesterol absorption. II. Changes in free and esterified cholesterol pools of mucosa after feeding cholesterol-4-C14.

In a previous study (1) it was shown that the transfer of fed cholesterol-4-Cl4 to the lymph was very rapid; the peak of absorption occurred in 6 to 8 hours, but cholesterol-4-Cl4 continued to appear in lymph up to 48 hours. There was considerable endogenous dilution of the fed cholesterol-4-Cl4 during its passage from the lumen to the lymph; this dilution was smallest during the period of rapi...

Differential uptake and metabolism of free and esterified cholesterol from high-density lipoproteins in the ovary.

Rat luteal cells utilize high-density lipoproteins (HDL) as a source of cholesterol for steroid synthesis. Both the free and esterified cholesterol of HDL are utilized by these cells. In this report, we have examined the relative uptake of free and esterified cholesterol of HDL by cultured rat luteal cells. Incubation of the cells with HDL labeled with [3H]cholesterol or [3H]cholesteryl linolea...

In vivo influx of free and esterified plasma cholesterol into human aortic tissue without atherosclerotic lesions.

In order to determine the in vivo influx of plasma cholesterol into human aortic intimamedia tissue, specimens of the ascending aortic wall without visible atherosclerosis were obtained from patients undergoing aortic valve replacement. Before the operation the patients were intravenously injected with autologous plasma in which the lipoproteins were labeled with radioactive cholesterol. The in...

Rapid method for determining free and esterified cholesterol in plasma extracts.

Stimulation of esterified cholesterol accumulation in tissue culture cells exposed to high density lipoproteins enriched in free cholesterol.

Human high density lipoprotein enriched in free cholesterol was obtained by exposing the lipoprotein to lipid dispersions having a free cholesterol/lecithin molar ratio greater than two. The metabolism of cholesterol was studied in tissue culture cells exposed to normal and cholesterol-enriched lipoproteins. Incubation of Fu5-AH rat hepatoma cells in medium containing cholesterol-enriched lipop...